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KMID : 0362719960340090667
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Journal of the Korean Dental Association
1996 Volume.34 No. 9 p.667 ~ p.687
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Release of Glutamate and GABA from Hippocampal Slice
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Abstract
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Present study was performed to clarify the release of glutamate and GABA employing hippocampal slices. Hippocampal slices(300-400μm thick) were prepared by the method of Kim et al. (1988) and pre-equilibrated in Krebs-bicarbonate medium (KBM, pH
7.4)
for 1hr at 37℃. Pre-equilibrate slices were incubated in fresh KBM and then potassium (50mM)-containing KBM for 5min period.
Basal and potassium-induced release of GABA and glutamic acid were determined from recovered medium by HPLC. After 30 min resting period, slices were reincubated in GABA plus potassium-containing medium consecutively for 5min period each to
investigate
the effect of GABA on basal or potassium-induced glutamic acid release from hippocampal slices.
@ES The observed results were as follows:
@EN 1. The release of glutamic acid induced by the first and second 5min-exposure of 50mM potassium was 143.2±15.6n mol and 115.1±9.2n mol, respectively. when compared with released amounts of glutamic acid during the corresponding spontaneous
periods these were 5.2 and 5.4 fold increase respectively.
2. GABA (0.01-1mM) inhibited potassium-induced glutamic acid release in dose-dependent manner.
3. Glutamic acid (0.1-10mM) enhanced spontaneous GABA release in dose-dependent manner.
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